The staining procedure involves several steps: 1. Fixation: The tissue sample is fixed to preserve its structure. 2. Impregnation: The sample is treated with silver nitrate, which impregnates the reticular fibers. 3. Reduction: A reducing agent is applied to convert the impregnated silver nitrate into metallic silver. 4. Toning: The sample is treated with a toner to enhance the visibility of the stained fibers. 5. Counterstaining: A counterstain may be applied to provide contrast between the stained fibers and other tissue components.
