methylation analysis

What Techniques are Used for Methylation Analysis?

Several techniques are employed for methylation analysis:
- Bisulfite Sequencing: This is the gold standard for detailed methylation mapping. It involves bisulfite treatment followed by sequencing.
- Methylation-Specific PCR (MSP): This technique amplifies bisulfite-treated DNA with primers specific for methylated or unmethylated DNA.
- Pyrosequencing: A quantitative method that provides sequencing data on the methylation status of CpG sites.
- Microarray-Based Methods: These include platforms like the Illumina Infinium MethylationEPIC BeadChip, which can assess methylation at a genome-wide scale.

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