What Techniques are Used for Methylation Analysis?
Several techniques are employed for methylation analysis: - Bisulfite Sequencing: This is the gold standard for detailed methylation mapping. It involves bisulfite treatment followed by sequencing. - Methylation-Specific PCR (MSP): This technique amplifies bisulfite-treated DNA with primers specific for methylated or unmethylated DNA. - Pyrosequencing: A quantitative method that provides sequencing data on the methylation status of CpG sites. - Microarray-Based Methods: These include platforms like the Illumina Infinium MethylationEPIC BeadChip, which can assess methylation at a genome-wide scale.