Neurotransmitter indicators are applied through various techniques to visualize neurotransmitters in tissue samples:
Immunohistochemistry (IHC): This method uses antibodies specific to neurotransmitters or their synthetic enzymes. These antibodies are conjugated to fluorescent or chromogenic markers, enabling visualization under a microscope. In Situ Hybridization (ISH): ISH involves the use of labeled complementary DNA or RNA probes to detect specific neurotransmitter mRNA within tissue sections, offering insights into the expression patterns of neurotransmitter-related genes. Fluorescence In Situ Hybridization (FISH): A variant of ISH, FISH uses fluorescent probes and is especially useful for detecting multiple neurotransmitters simultaneously.
