Fixation: The tissue is immersed in a formalin solution, usually 10% neutral-buffered formalin, for several hours to days. This step preserves the tissue by cross-linking proteins and preventing degradation. Dehydration: The fixed tissue is then dehydrated through a series of graded alcohol baths, typically ethanol, to remove water content. Clearing: The tissue is cleared using a solvent like xylene, which makes it easier for the paraffin to infiltrate the tissue. Embedding: The cleared tissue is then infiltrated with molten paraffin wax and allowed to harden, forming a solid block. Sectioning: Thin sections, usually 4-5 micrometers thick, are cut from the paraffin block using a microtome. These sections are then mounted on glass slides for staining and analysis.
