In histology, the study of neurochemicals involves various techniques to visualize and quantify these substances within tissues. Common methods include:
Immunohistochemistry (IHC): This technique uses antibodies to detect specific neurochemicals in tissue sections. In situ hybridization (ISH): This method detects the mRNA expression of neurochemicals, providing insights into their synthesis. Fluorescence microscopy: This allows for the visualization of neurochemicals with fluorescent tags. Enzyme-linked immunosorbent assay (ELISA): This is used to quantify specific neurochemicals within a tissue sample.