How are Double Strand Breaks Detected in Histology?
In histology, DSBs can be detected using techniques like immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). These techniques can identify markers of DSBs, such as phosphorylated H2AX (γ-H2AX) and 53BP1. The presence of these markers indicates the activation of DNA damage response pathways, providing insights into the extent and repair of DSBs in tissue samples.
