How are Cytokines Detected and Measured in Histological Studies?
In histological studies, cytokines can be detected and quantified using various techniques such as:
- Immunohistochemistry (IHC): This method uses antibodies specific to cytokines to visualize their presence and distribution in tissue sections. - In situ hybridization (ISH): Detects cytokine mRNA within tissue sections, providing information on cytokine gene expression. - Enzyme-linked immunosorbent assay (ELISA): Although not a histological technique, ELISA can be used to quantify cytokines in tissue homogenates or culture supernatants.
