Why is Tween 20 important in Immunohistochemistry?
In immunohistochemistry, Tween 20 is crucial for blocking non-specific binding sites. It can be included in blocking buffers to reduce background staining, ensuring that antibodies bind specifically to their target antigens. This specificity is vital for producing clear and interpretable results. Tween 20 also enhances the washing steps, removing unbound antibodies and reducing non-specific signals.
