In histology, Tween 20 is commonly used as a detergent and wetting agent. It helps in the permeabilization of cell membranes, allowing for better penetration of stains and antibodies during immunohistochemistry (IHC) and immunofluorescence (IF) techniques. By breaking down lipid barriers, Tween 20 facilitates the entry of these molecules into the cells and tissues, leading to more effective and accurate staining.
