Fluorometry is crucial in histology for several reasons:
Sensitivity: Fluorometry is highly sensitive, capable of detecting low concentrations of fluorescently labeled molecules. Specificity: It allows for the specific labeling and detection of particular cellular components, such as proteins, nucleic acids, and lipids. Quantification: This technique provides quantitative data, enabling researchers to measure the concentration of specific molecules within tissues. Spatial Resolution: Fluorometry can be combined with microscopy techniques to provide high-resolution images of tissue structures.
