What are the Applications of Fluorometry in Histology?
Fluorometry has a wide range of applications in histology, including:
Immunohistochemistry (IHC): This technique uses antibodies conjugated with fluorophores to detect specific antigens in tissue sections. Fluorescence In Situ Hybridization (FISH): FISH uses fluorescent probes to detect and localize specific DNA sequences within chromosomes. Live Cell Imaging: Fluorometry enables the observation of live cells and tissues in real-time, providing insights into dynamic cellular processes. Cellular and Subcellular Localization: It helps in identifying the location of specific molecules within cells and tissues.