Detachment can occur due to several reasons: - Mechanical Stress: During tissue processing, handling, or sectioning, mechanical forces can cause cells or tissues to detach from the substrate. - Enzymatic Digestion: Enzymes used in certain histological procedures can degrade the extracellular matrix, leading to detachment. - Chemical Agents: Some chemicals used in staining or fixation may weaken the adhesion between cells and the substrate. - Cell Death: Necrosis or apoptosis can lead to the detachment of cells from the tissue matrix.
