The process of culturing cells involves several critical steps:
Isolation of Cells: Cells are isolated from tissues using enzymatic or mechanical methods. Culture Medium: Cells are placed in a nutrient-rich medium that provides essential growth factors, hormones, and nutrients. Incubation: The cultured cells are incubated at optimal conditions, including temperature, humidity, and CO2 levels. Sub-Culturing: When cells reach confluency, they are passaged or sub-cultured to provide more space and nutrients.
