Preventing detachment is crucial for maintaining the integrity of histological samples. Some strategies include: - Optimizing Fixation: Proper fixation using agents like formaldehyde can help preserve tissue structure and prevent detachment. - Careful Handling: Gentle handling and minimizing mechanical stress during processing and sectioning can reduce the risk of detachment. - Adhesive Substrates: Using positively charged slides or adhesive coatings can enhance cell adhesion. - Controlled Enzymatic Digestion: Using optimal enzyme concentrations and incubation times can prevent excessive tissue degradation.
