Negative controls are crucial for validating the results of histological staining. They help in:
Ensuring Specificity: By showing no staining, negative controls confirm that the staining observed in the test samples is specific to the target antigen. Identifying Non-specific Binding: They help identify non-specific binding of reagents to tissue sections. Confirming Reagent Functionality: They verify that the reagents used in the staining procedure are functioning correctly and not contributing to background staining.
