Buffer solutions are crucial in histology for several reasons:
Stability of pH: Biological tissues are sensitive to changes in pH. Maintaining a constant pH ensures that the tissue morphology and the activity of enzymes remain unaffected during processing. Optimal Staining: Many histological stains require specific pH levels to bind effectively to cellular components. Buffer solutions help achieve and maintain these pH levels. Preservation of Tissue Integrity: By preventing drastic pH changes, buffer solutions help preserve the structural integrity of tissues, which is essential for accurate microscopic analysis.
