Several histological techniques specifically require fresh tissue:
1. Enzyme Histochemistry: This technique studies enzyme activity in tissues, requiring fresh samples to prevent enzyme degradation. 2. Immunohistochemistry (IHC): Fresh tissue ensures optimal antigen preservation for antibody binding. 3. Fluorescence Microscopy: Fresh tissue can be stained with fluorescent dyes to study cellular structures and functions. 4. Electron Microscopy: Although often fixed, some electron microscopy techniques benefit from fresh tissue to observe ultrastructural details. 5. Molecular Techniques: Fresh tissue is ideal for isolating RNA, DNA, and proteins for molecular biology studies.
