What Techniques Are Used to Study Transformation in Histology?
Several techniques are employed to study cellular transformation in histology:
Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins in tissue sections, allowing for the identification of transformed cells. In situ hybridization (ISH): This method detects specific nucleic acid sequences within tissues, providing insights into gene expression changes during transformation. Flow cytometry: This technique analyzes the physical and chemical characteristics of cells, including cell size, granularity, and protein expression, to identify transformed cells. Next-generation sequencing (NGS): This advanced technique allows for comprehensive analysis of genetic changes associated with transformation.
