What Techniques are Used to Study Serotonergic Systems in Histology?
Several techniques are employed to study serotonergic systems in histology. Immunohistochemistry (IHC) is commonly used to localize serotonin and its receptors in tissues by using specific antibodies. In situ hybridization (ISH) can detect the mRNA expression of serotonin receptors. Electron microscopy (EM) might be used to observe the ultrastructural details of serotonergic neurons and synapses. Additionally, various fluorescence microscopy techniques can visualize serotonin and its pathways in live tissues.
