What Techniques are Used to Study Notch Signaling in Histology?
Several techniques are employed to study Notch signaling in the context of histology:
Immunohistochemistry (IHC): Used to detect Notch receptors and ligands in tissue sections. In Situ Hybridization (ISH): Used to localize specific mRNA transcripts of Notch pathway components. Western Blotting: Used to detect and quantify Notch proteins and their cleavage products. Confocal Microscopy: Allows for detailed visualization of Notch signaling components in cellular contexts.
