What techniques are used to study NF-κB in histology?
Several techniques are employed to study NF-κB in histological contexts:
Immunohistochemistry (IHC): Used to detect NF-κB subunits in tissue sections by using specific antibodies. Western Blotting: Measures the expression levels of NF-κB subunits in tissue extracts. Electrophoretic Mobility Shift Assay (EMSA): Assesses DNA-binding activity of NF-κB. Chromatin Immunoprecipitation (ChIP): Used to determine the binding of NF-κB to specific DNA regions in the chromatin context.
