What Techniques are Used to Study Microenvironmental Heterogeneity?
Several advanced techniques are employed to study microenvironmental heterogeneity:
Immunohistochemistry (IHC): This technique uses antibodies to detect specific antigens in tissue sections, providing insights into the spatial distribution of proteins. In Situ Hybridization (ISH): ISH allows for the localization of specific nucleic acid sequences within tissue sections, helping to identify gene expression patterns. Multiplexed Imaging: Techniques like multiplexed IHC and mass cytometry enable the simultaneous detection of multiple markers, providing a detailed view of tissue heterogeneity. Single-Cell Sequencing: This technique allows for the analysis of gene expression at the single-cell level, uncovering cellular diversity within tissues.
