What Techniques are Used to Study APP in Histology?
Several histological techniques are employed to study APP, including:
Immunohistochemistry (IHC): This technique uses antibodies specific to APP to visualize its distribution in tissue sections. IHC can reveal the localization of APP in neuronal synapses and other cell types. Western Blotting: This method is used to detect APP and its cleavage products in tissue extracts, providing quantitative information about the expression levels of APP. In situ Hybridization: This technique detects APP mRNA, offering insights into the gene expression patterns of APP in various tissues.
