Several advanced techniques are employed in in vivo imaging:
Fluorescence Microscopy: Involves the use of fluorescent dyes or proteins to label specific cellular components, which are then visualized using specialized microscopes. Confocal Microscopy: Enhances the resolution and contrast of fluorescence images by using a spatial pinhole to eliminate out-of-focus light. Multiphoton Microscopy: Allows deep tissue imaging by using long-wavelength light, minimizing photodamage and phototoxicity. Optical Coherence Tomography (OCT): Provides high-resolution cross-sectional images of tissues using light waves. Magnetic Resonance Imaging (MRI): Utilizes magnetic fields and radio waves to create detailed images of organs and tissues.
