Several advanced techniques have been developed for deep tissue imaging. Among these:
- Confocal Microscopy: This technique uses point illumination and a spatial pinhole to eliminate out-of-focus light, allowing for clearer images of deep tissue sections. - Two-Photon Excitation Microscopy: It employs two photons of lower energy to excite fluorophores, reducing phototoxicity and enabling deeper tissue penetration. - Optical Coherence Tomography (OCT): OCT provides high-resolution cross-sectional images of tissues using light waves, suitable for imaging depths of a few millimeters. - Light Sheet Fluorescence Microscopy (LSFM): This method illuminates the sample with a thin sheet of light, allowing for rapid and high-contrast imaging of thick samples.
