Several techniques are employed for organelle isolation, each with its own advantages and limitations:
Differential Centrifugation: This method separates organelles based on their size and density through a series of centrifugation steps. Density Gradient Centrifugation: Organelles are separated in a gradient medium, such as sucrose or Percoll, based on their buoyant density. Immunoprecipitation: This technique uses antibodies that specifically bind to proteins on the surface of the target organelle. Magnetic Bead Isolation: Employs magnetic beads coated with specific antibodies to capture and isolate desired organelles.
