What Staining Techniques are Used to Study Osteons?
Several staining techniques are employed in histology to study osteons, including:
Hematoxylin and Eosin (H&E): This common staining method highlights the general structure of bone tissue, with hematoxylin staining nuclei blue and eosin staining the cytoplasm and extracellular matrix pink. Masson's Trichrome: This technique distinguishes collagen fibers (stained blue or green) from other tissue components, providing detailed visualization of lamellae. Von Kossa Stain: This method specifically stains calcium deposits, making it useful for identifying areas of mineralization within the bone matrix.
