What Staining Techniques Are Used to Identify Apoptotic Cells?
Several staining techniques are used to identify apoptotic cells: - Hematoxylin and Eosin (H&E) Staining: Provides general morphological details. - TUNEL Assay: Detects DNA fragmentation by labeling the terminal end of nucleic acids. - Annexin V Staining: Binds to phosphatidylserine exposed on the outer leaflet of the plasma membrane in early apoptosis. - Caspase Activity Assays: Detects activated caspases, enzymes that play a crucial role in apoptosis.
