What Staining Techniques are Used to Detect Fatty Casts?
Several staining techniques can be employed to enhance the visualization of fatty casts in histological specimens:
1. Sudan III or IV Stain: These lipid-specific dyes stain the fat droplets within the casts, imparting an orange-red color. 2. Oil Red O Stain: Another lipid-specific stain that provides a red coloration to the fat droplets. 3. Hematoxylin and Eosin (H&E) Stain: While not specific for lipids, H&E staining can help identify the overall structure of the cast and its cellular components.