Despite the strong evidence supporting the endosymbiotic theory, several questions remain unanswered. For example, the exact mechanisms by which the original symbiotic relationship developed and evolved into the permanent integration seen today are not fully understood. Additionally, while mitochondria and chloroplasts are well-established examples, it is hypothesized that other organelles, such as the peroxisomes, may also have endosymbiotic origins, but this is still under investigation. Understanding these mechanisms could provide deeper insights into the evolution of cellular complexity.