The Dopa Reaction involves incubating tissue sections with a solution of L-DOPA. The steps are as follows: 1. Preparation of the tissue section: Tissue samples are fixed, usually in formalin, and then embedded in paraffin. Thin sections are cut using a microtome and placed on slides. 2. Incubation with L-DOPA: The tissue sections are incubated with a solution containing L-DOPA. Tyrosinase present in the tissue oxidizes L-DOPA to DOPAquinone, which further polymerizes to form melanin-like pigments. 3. Examination under a microscope: The sections are washed, dehydrated, and coverslipped for examination. The presence of melanin is identified as dark brown or black deposits under the microscope.
