During the citric acid cycle, NADH and FADH2 are produced through the oxidation of intermediates. These molecules serve as electron carriers and donate electrons to the electron transport chain (ETC), located in the inner mitochondrial membrane. The ETC uses these electrons to drive the production of ATP through oxidative phosphorylation. In histology, the activity of the ETC can be assessed using specific histochemical techniques, providing insights into mitochondrial function and tissue health.
