Fixation: Preserving the tissue in a fixative like formalin to prevent decay and maintain its structure. Embedding: Enclosing the tissue in a medium like paraffin to provide support during slicing. Trimming: Cutting away excess embedding medium to expose the tissue surface. Sectioning: Using a microtome to slice the tissue into thin sections, typically 3-5 micrometers thick. Mounting: Placing the sections onto glass slides for staining and examination.
