Several challenges can affect the quality of sectioning:
Tissue Hardness: Hard tissues like bone require special preparation techniques, such as decalcification, to be sectioned properly. Blade Sharpness: Dull blades can cause tearing and uneven sections. Regular replacement and sharpening of blades are essential. Temperature Control: For cryostat sectioning, maintaining an appropriate temperature is crucial to prevent tissue damage. Embedding Medium: Improper embedding can lead to tissue distortion. It is important to choose the right medium and ensure complete infiltration.
