What is the Principle of Immunohistochemistry (IHC)?
Immunohistochemistry (IHC) is a technique that uses antibodies to detect specific antigens in tissues. The primary antibody binds to the target antigen, and a secondary antibody, conjugated with an enzyme or fluorophore, binds to the primary antibody. This creates a colored or fluorescent signal, indicating the presence and location of the antigen. IHC is valuable for diagnostic pathology and research.