NADH diaphorase staining is a histological technique used to identify and visualize the activity of NADH (Nicotinamide Adenine Dinucleotide, reduced form) dehydrogenase enzymes in tissue sections. These enzymes play a crucial role in cellular respiration, particularly in the mitochondria. The staining method is especially useful for examining muscle tissues, liver, and other organs where oxidative metabolism is significant.