How is Calcium Overload Detected in Histological Samples?
Several techniques can be employed to detect calcium overload in histological samples:
1. Calcium Staining: Stains such as Alizarin Red S and Von Kossa can be used to visualize calcium deposits. 2. Electron Microscopy: Provides detailed images of subcellular structures affected by calcium overload. 3. Fluorescent Indicators: Calcium-sensitive dyes like Fura-2 can be used to measure intracellular calcium levels.