What histological techniques are used to study these changes?
Several histological techniques can be employed to study the effects of corticosteroids and their discontinuation:
- Hematoxylin and Eosin (H&E) Staining: This is a primary technique for general tissue morphology. - Immunohistochemistry (IHC): Used to detect specific proteins that may indicate cellular responses or inflammation. - Masson's Trichrome Staining: Useful for assessing collagen and connective tissue changes. - Electron Microscopy: Provides detailed images of cellular ultrastructure.
