What Techniques are Used to Study the Effects of Environmental Toxins?
Several histological techniques are employed to study the effects of environmental toxins. Histochemical staining methods such as Hematoxylin and Eosin (H&E) staining can reveal general tissue architecture and cell morphology. Specific stains like Prussian blue can detect iron deposits, indicating oxidative damage. Immunohistochemistry (IHC) can be used to identify specific proteins and markers of cell damage, apoptosis, and proliferation. Electron microscopy provides detailed images of cellular ultrastructure, helping to identify sub-cellular damage caused by toxins.
