What Histological Techniques are Used in Air Pollution Studies?
Several histological techniques are employed to study the impact of air pollution on tissues:
1. Hematoxylin and Eosin (H&E) Staining: This is a routine staining method used to observe general tissue morphology and identify structural changes. 2. Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins associated with inflammation and cellular stress. 3. Electron Microscopy: Provides high-resolution images to study ultrastructural changes in cells and tissues. 4. Special Stains: Techniques such as Masson's Trichrome and Periodic Acid-Schiff (PAS) staining are used to identify fibrosis and mucous secretions, respectively.