Once the bone sample is obtained, it is sent to a histology lab for processing. The tissue undergoes several steps: - Fixation: The sample is preserved using chemical fixatives like formaldehyde to prevent decay. - Decalcification: Since bone is highly calcified, it needs to be softened using acids or chelating agents. - Embedding: The soft tissue is embedded in paraffin wax to provide support for slicing. - Sectioning: Thin slices of the tissue are cut using a microtome. - Staining: The sections are stained with histological stains like Hematoxylin and Eosin (H&E) to differentiate cellular components.
