How is DIF different from Indirect Immunofluorescence (IIF)?
In Indirect Immunofluorescence (IIF), a primary antibody binds to the antigen, and a secondary antibody that is conjugated to a fluorescent dye binds to the primary antibody. This two-step process can amplify the signal but may also introduce more background noise. In contrast, DIF involves the direct binding of the fluorescently labeled primary antibody to the antigen, making the procedure faster and reducing the risk of non-specific staining.
