What are the Tools Used to Study Calcium Signaling in Histology?
Various tools are used to study calcium signaling in histology. Fluorescent dyes like Fura-2 and Fluo-4 are commonly used to visualize calcium levels in cells. These dyes bind to Ca2+ and emit fluorescence when excited by specific wavelengths of light. Advanced imaging techniques such as confocal microscopy and two-photon microscopy are also employed to obtain high-resolution images of calcium dynamics within cells and tissues.