What Factors Should Be Considered in Probe Design?

Several factors should be considered during probe design to ensure optimal performance:

1. Target Specificity: The probe must specifically bind to the target molecule without cross-reacting with other molecules.
2. Probe Length: The length of the probe should be optimized for stability and binding efficiency.
3. Labeling: The probe must be labeled with a detectable marker, such as a fluorescent dye or an enzyme.
4. Hybridization Conditions: The conditions under which the probe hybridizes to the target must be optimized for temperature, salt concentration, and other parameters.
5. Accessibility: The target region must be accessible to the probe within the tissue sample.