Despite the advancements in probe design, several challenges remain:
1. Non-Specific Binding: Designing probes that avoid non-specific interactions with non-target molecules. 2. Tissue Penetration: Ensuring that probes can penetrate tissue samples effectively. 3. Signal-to-Noise Ratio: Maximizing the signal from the target molecule while minimizing background noise. 4. Probe Stability: Ensuring that probes remain stable and functional under experimental conditions.
