Several factors influence the compatibility of substrates in histology:
Type of Tissue: Different tissues have varying requirements. For example, bone tissues may need substrates that support decalcification, while soft tissues require substrates that support their delicate structure. Staining Techniques: Some staining protocols are sensitive to the chemical composition of the substrate. For instance, Hematoxylin and Eosin (H&E) staining may require different substrate properties compared to immunohistochemistry. Fixation Methods: The type of fixation (e.g., formalin, alcohol) can interact with the substrate, affecting tissue adherence and staining. Environmental Conditions: Factors such as temperature, humidity, and exposure to light can influence substrate performance.