Gene knock-in is typically achieved using homologous recombination or CRISPR-Cas9 technology. Homologous recombination involves introducing DNA sequences that are homologous to target genome sequences, facilitating precise insertion. CRISPR-Cas9, on the other hand, uses a guide RNA to direct the Cas9 enzyme to a specific genomic locus, where it induces a double-strand break, allowing for the insertion of the desired gene.
