1. Delayed Fixation: Prolonged time between tissue removal and fixation can lead to autolysis and degradation. 2. Improper Fixation: Using the wrong type or concentration of fixative can alter tissue morphology. 3. Temperature Variations: Fluctuations in temperature during storage and transport can damage tissues. 4. Mechanical Damage: Rough handling can tear or distort delicate tissues. 5. Contamination: Introduction of foreign substances can obscure or alter tissue features.
