Several factors can influence signal intensity, including:
Staining protocols: The type and concentration of stains or dyes used can significantly impact the signal intensity. Tissue preparation: The method of tissue fixation, embedding, and sectioning can affect the quality and intensity of the signal. Imaging parameters: The settings on the imaging device, such as exposure time, aperture, and gain, can alter signal intensity. Fluorophore properties: The inherent brightness and photostability of fluorophores used in fluorescence microscopy play a significant role in determining signal intensity.
