Sample Preparation: Tissue samples are homogenized and proteins are extracted. Isoelectric Focusing (IEF): Proteins are separated in a pH gradient based on their isoelectric point. Equilibration: Focused proteins are treated with SDS to impart a uniform negative charge. SDS-PAGE: Proteins are further separated based on their molecular weight. Detection: Proteins are visualized using stains like Coomassie Blue or silver staining.
